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Src antibody (AA 24-52)

This Rabbit Polyclonal antibody specifically detects Src in WB, IHC, ELISA, IF and FACS. It exhibits reactivity toward Human.

Src antibody western blot analysis in (1) MCF-7 and (2) A431 lysate (Src antibody (AA 24-52))

Confocal immunofluorescent analysis of Src antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Src antibody (AA 24-52))

Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue (Src antibody (AA 24-52))

Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue (Src antibody (AA 24-52))

Confocal immunofluorescent analysis of Src antibody with A431 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Src antibody (AA 24-52))

Src antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue (Src antibody (AA 24-52))

Confocal immunofluorescent analysis of Src antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Src antibody (AA 24-52))

Confocal immunofluorescent analysis of Src antibody with A431 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Src antibody (AA 24-52))

Src antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control (left histogram). (Src antibody (AA 24-52))

Src antibody western blot analysis in (1) MCF-7 and (2) A431 lysate (Src antibody (AA 24-52))

10 images

Catalog No. ABIN3029554

Quick Overview for Src antibody (AA 24-52) (ABIN3029554)

Target

See all Src Antibodies

Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity

262
172
170
9
7
7
5
5
4
4
4
2
1
1
1

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

123
23
15
12
8
8
8
8
8
8
8
7
6
6
4
4
4
4
4
4
4
4
4
4
4

This Src antibody is un-conjugated

Application

236
114
114
76
56
55
53
29
27
23
12
9
4
3
1

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Discover our top product Src Primary Antibody

Binding Specificity
32

25

25

19

17

15

15

12

10

8

7

6

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 24-52

Cross-Reactivity (Details)

Expected species reactivity: Mouse

Purification

Antigen affinity purified

Immunogen

A portion of amino acids 24-52 from the human protein was used as the immunogen for this Src antibody.

Isotype

Ig Fraction
anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
Src Reactivity: Human, Mouse WB, IF Host: Mouse Monoclonal 17AT28 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 24-52), (N-Term) antibody
Src Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB41141 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
Src Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 1F11 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (pTyr530) antibody
Src Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
KO Validated Src Reactivity: Human WB, IF, IP Host: Rabbit Monoclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 397-424) antibody
Src Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (pTyr529) antibody
Src Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (pTyr419) antibody
Src Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 397-424) antibody
Src Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB21492 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 1-90) antibody
Src Reactivity: Human WB, ELISA, IF, IHC (p), PLA Host: Mouse Monoclonal 1B9 unconjugated

Application Notes

Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:2000,IHC (Paraffin): 1:50,Flow Cytometry: 1:50,Immunofluorescence: 1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name

Src

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

UniProt

P12931

Pathways

JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

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